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Anti-CYP7A1 Antibody (A8675)

Western Blot - Anti-CYP7A1 Antibody (A8675) - Antibodies.com Immunofluorescence - Anti-CYP7A1 Antibody (A8675) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CYP7A1 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-CYP7A1 Antibody
Description
Rabbit polyclonal antibody to CYP7A1.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 390-504 of human CYP7A1 (NP_000771.2).
Sequence
YPQLMHLDPEIYPDPLTFKYDRYLDENGKTKTTFYCNGLKLKYYYMPFGSGATICPGRLFAIHEIKQFLILMLSYFELELIEGQAKCPPLDQSRAGLGILPPLNDIEFKYKFKHL
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
58 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Cholesterol 7 alpha hydroxylase, Cholesterol 7 alpha monooxygenase, Cholesterol 7-alpha-hydroxylase, Cholesterol 7-alpha-monooxygenase, CP7A, CP7A1_HUMAN, CYP 7, CYP7, CYPVII, Cytochrome P450 7A1, Cytochrome P450, family 7, subfamily A, polypeptide 1, Cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase),, Cytochrome P450, subfamily VIIA (cholesterol 7 alpha-monooxygenase), polypeptide 1, MGC126826, MGC138389
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CYP7A1 Antibody (A8675) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CYP7A1 Antibody (A8675)
Western blot analysis of extracts of various cell lines, using Anti-CYP7A1 Antibody (A8675) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - Anti-CYP7A1 Antibody (A8675) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-CYP7A1 Antibody (A8675)
Immunofluorescence analysis of HepG2 cells using Anti-CYP7A1 Antibody (A8675) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-CYP7A1 Antibody (A8675)? Please let us know so that we can list the citation on this page.

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