Western Blot - Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326) at 1:1,000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.
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