Rabbit monoclonal [ARC1559] antibody to Cyclin E1 (phospho Thr77).
Applications
WB
Dilutions
WB: 1:500-1:2,000
Reactivity
Human, Mouse
Immunogen
A synthetic phosphorylated peptide around T77 of human Cyclin E1 (P24864).
Sequence
IPTPD
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC1559
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity purification.
Molecular Weight
54 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
Figure 1: Western Blot - Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326)
Western blot analysis of extracts of NIH/3T3 cells, using Anti-Cyclin E1 (phospho Thr77) Antibody [ARC1559] (A306326) at 1:1,000 dilution. NIH/3T3 cells were treated by Hydroxyurea (4 Mm) at 37°C for 20 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 3min.
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