Rabbit polyclonal antibody to Cyclin E1 (phospho Thr77).
Specificity
This antibody detects endogenous levels of Cyclin E1 only when phosphorylated at Thr77.
Applications
IF, ELISA
Dilutions
WB: 1:500-1:1000, IF: 1:100-1:500, ELISA: 1:40000
Reactivity
Human
Immunogen
Synthetic peptide derived from human Cyclin E1 around the phosphorylation site of Thr77 (amino acids 43-92).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing peptide.
Molecular Weight
47kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
Immunofluorescence analysis of HeLa cells using Anti-Cyclin E1 (phospho Thr77) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.
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