Anti-Cyclin E1 Antibody [rCCNE1/4936] - BSA and Azide free
Description
Recombinant mouse monoclonal [rCCNE1/4936] antibody to Cyclin E1.
Specificity
Cyclin E belongs to the highly conserved cyclin family, whose members exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclins function as regulators of CDK kinases. Cyclin E forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. Cyclin E accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Cyclin E overexpression has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
Applications
WB, IHC-P
Dilutions
WB: 1-2 µg/ml, IHC-P: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant fragment, around amino acids 10-176, of human Cyclin E1. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
rCCNE1/4936
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
53 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1
SDS-PAGE analysis of Anti-Cyclin E1 Antibody [rCCNE1/4936] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.
Figure 4: Western Blot - Anti-Cyclin E1 Antibody [rCCNE1/4936] - BSA and Azide free (A278519)
Western blot analysis of HCT116 cell line lysate using Anti-Cyclin E1 Antibody [rCCNE1/4936].
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