Anti-Cyclin E1 Antibody [ARC51383] (A305272)

Name: Anti-Cyclin E1 Antibody [ARC51383]
Brand: Antibodies.com
SKU: A305272
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC51383] antibody to Cyclin E1 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Cyclin E1 Antibody [ARC51383]

Description

Rabbit monoclonal [ARC51383] antibody to Cyclin E1.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human Cyclin E1 (NP_001229.1).

Sequence

AASALYHFSSSELMQKVSGYQWCDIENCVKWMVPFAMVIRETGSSKLKHFRGVADEDAHNIQTHRDSLDLLDKARAKKAMLSEQNRASPLPSGLLTPPQSG

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC51383

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

48 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CCNE, Ccne1, CCNE1_HUMAN, cyclin E variant ex5del, cyclin E variant ex7del, Cyclin Es, Cyclin Et, CyclinE, G1/S specific cyclin E, G1/S-specific cyclin-E1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-Cyclin E1 Antibody [ARC51383] (A305272)

Western blot analysis of Mouse spleen, using Anti-Cyclin E1 Antibody [ARC51383] (A305272) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Immunohistochemistry - Anti-Cyclin E1 Antibody [ARC51383] (A305272)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Cyclin E1 Antibody [ARC51383] (A305272) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Cyclin E1 Antibody [ARC51383] (A305272)

Immunohistochemistry analysis of paraffin-embedded human brain (negative control sample) using Anti-Cyclin E1 Antibody [ARC51383] (A305272) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Cyclin E1 Antibody [ARC51383] (A305272)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-Cyclin E1 Antibody [ARC51383] (A305272) at a dilution of 1:100(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.