Rabbit polyclonal antibody to Cyclin D2 (Phospho-Thr280)
Specificity
The antibody detects endogenous levels of Cyclin D2 only when phosphorylated at threonine 280.
Applications
WB
Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of threonine 280 (A-S-T(p)-P-T) derived from Human Cyclin D2.
Host
Rabbit
Clonality
Polyclonal
Conjugate
Unconjugated
Purification
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Concentration
1.0mg / ml
Formulation
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Western blot analysis of extracts from COLO cells, treated with EGF (200ng/ml, 30mins), using Cyclin D2 (Phospho-Thr280) antibody #12122. The lane on the right is treated with the synthesized peptide.
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