Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Name: Anti-Cyclin D1 Antibody [ARC0300]
Brand: Antibodies.com
SKU: A306339
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0300] antibody to Cyclin D1 for WB, IHC, ICC/IF and IP.

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Knockout Validated Antibody

Specifications

Name

Anti-Cyclin D1 Antibody [ARC0300]

Description

Rabbit monoclonal [ARC0300] antibody to Cyclin D1.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 196-295 of human Cyclin D1 (P24385).

Sequence

ISNPPSMVAAGSVVAAVQGLNLRSPNNFLSYYRLTRFLSRVIKCDPDCLRACQEQIEALLESSLRQAQQNMDPKAAEEEEEEEEEVDLACTPTDVRDVDI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0300

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

34 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AI327039, B cell CLL/lymphoma 1, B cell leukemia 1, B cell lymphoma 1 protein, B-cell lymphoma 1 protein, BCL 1, BCL-1, BCL-1 oncogene, BCL1, BCL1 oncogene, ccnd1, CCND1/FSTL3 fusion gene, included, CCND1/IGHG1 fusion gene, included, CCND1/IGLC1 fusion gene, included, CCND1/PTH fusion gene, included, CCND1_HUMAN, cD1, Cyl 1, D11S287E, G1/S specific cyclin D1, G1/S-specific cyclin-D1, Parathyroid adenomatosis 1, PRAD1, PRAD1 oncogene, U21B31

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (10)

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Figure 1: Western Blot - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Western blot analysis of extracts from wild type (WT) and Cyclin D1 knockout (KO) HeLa cells, using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 2: Western Blot - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Western blot analysis of extracts of various cell lines, using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1min.

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Figure 3: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 5: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 6: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 7: Immunohistochemistry - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunohistochemistry analysis of paraffin-embedded mouse liver using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 8: Immunofluorescence - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunofluorescence analysis of C6 cells using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 9: Immunofluorescence - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunofluorescence analysis of NIH-3T3 cells using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 10: Western Blot - Anti-Cyclin D1 Antibody [ARC0300] (A306339)

Immunoprecipitation analysis of 300µg extracts of MCF7 cells using 3µg of Anti-Cyclin D1 Antibody [ARC0300] (A306339). This Western blot was performed on the immunoprecipitate using Anti-Cyclin D1 Antibody [ARC0300] (A306339) at a dilution of 1:1000.

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