Anti-Cyclin B1 Antibody (A90462)

Name: Anti-Cyclin B1 Antibody
Brand: Antibodies.com
SKU: A90462
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to Cyclin B1 for WB, IHC and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-Cyclin B1 Antibody

Description

Rabbit polyclonal antibody to Cyclin B1.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 350-433 of human CCNB1 (NP_114172.1).

Sequence

CLALKILDNGEWTPTLQHYLSYTEESLLPVMQHLAKNVVMVNQGLTKHMTVKNKYATSKHAKISTLPQLNSALVQDLAKAVAKV

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

55 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CCNB, CCNB 1, ccnb1, CCNB1_HUMAN, G2 mitotic specific cyclin B1, G2/mitotic-specific cyclin-B1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Cyclin B1 Antibody (A90462)

Western blot analysis of extracts from normal (control) and Cyclin B1 knockout (KO) HeLa cells, using Anti-Cyclin B1 Antibody (A90462) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-Cyclin B1 Antibody (A90462)

Western blot analysis of extracts of Mouse liver, using Anti-Cyclin B1 Antibody (A90462) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-Cyclin B1 Antibody (A90462)

Immunohistochemistry analysis of paraffin-embedded rat ovary using Anti-Cyclin B1 Antibody (A90462) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Cyclin B1 Antibody (A90462)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Cyclin B1 Antibody (A90462) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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