Anti-CTCF Antibody [ARC0067] (A308115)

Name: Anti-CTCF Antibody [ARC0067]
Brand: Antibodies.com
SKU: A308115
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0067] antibody to CTCF for WB, ICC/IF, IP, ChIP and ChIP-seq.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-CTCF Antibody [ARC0067]

Description

Rabbit monoclonal [ARC0067] antibody to CTCF.

Applications

WB, ICC/IF, IP, ChIP, ChIP-seq

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000, ChIP: 1:20-1:100, CUT&Tag: 1⁶ cells/2µg, ChIP-seq: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 628-727 of human CTCF (P49711).

Sequence

PAVEIEPEPEPQPVTPAPPPAKKRRGRPPGRTNQPKQNQPTAIIQVEDQNTGAIENIIVEVKKEPDAEPAEGEEEEAQPAATDAPNGDLTPEMILSMMDR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0067

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

140 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

11-zinc finger protein, CCCTC-binding factor, CTCFL paralog, Transcriptional repressor CTCF

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Anti-CTCF Antibody [ARC0067] (A308115)

CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ K562 cells with 2µg of Anti-CTCF Antibody [ARC0067] (A308115), and Goat Anti-Rabbit IgG H&L Antibody. The CUT&Tag results indicate the enrichment pattern of CTCF in representative gene loci (MYC), as shown in figure.

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Chromatin Immunoprecipitation - Anti-CTCF Antibody [ARC0067] (A308115)

Chromatin immunoprecipitations analysis of cross-linked chromatin from 293T cells, using Anti-CTCF Antibody [ARC0067] (A308115). The ChIP sequencing results indicate the enrichment pattern of CTCF in selected genomic region and representative gene loci (MYC), as shown in figure.

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Western Blot - Anti-CTCF Antibody [ARC0067] (A308115)

Western blot analysis of extracts of various cell lines, using Anti-CTCF Antibody [ARC0067] (A308115) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.