Recombinant human monoclonal antibody to Glucagon Receptor.
Applications
ELISA, FACS, Functional Assay, In Vivo
Reactivity
Human, Mouse, Rat, Rhesus Macaque, Cynomolgus Macaque
Host
Human
Clonality
Monoclonal
Isotype
IgG4SP - Human IgG4 with S228P mutation
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A affinity chromatography.
Concentration
Reconstitution dependent.
Molecular Weight
This antibody has a predicted MW of 146.82 kDa.
Purity
> 95% (by SDS-PAGE and SEC-HPLC).
Product Form
Lyophilized
Reconstitution
Reconstitute with 100µl of sterile double-distilled water to bring antibody to 1mg/ml concentration. Gently shake to solubilize completely. Do not vortex!
Formulation
Lyophilized from 25mM Histadine, pH 6.2, with 8% Sucrose and 0.01% Tween80.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
SDS-PAGE analysis of Crotedumab Biosimilar - Anti-Glucagon Receptor Antibody - Low endotoxin, Azide free (A323374) under reducing (R) conditions confirms that the purity of this antibody is greater than 95%.
SEC-HPLC analysis of Crotedumab Biosimilar - Anti-Glucagon Receptor Antibody - Low endotoxin, Azide free (A323374) confirms that the purity of this antibody is 96.61%.
Human GCCR His EGFP HEK293 cells were stained with Crotedumab Biosimilar - Anti-Glucagon Receptor Antibody - Low endotoxin, Azide free (A323374) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC50 = 0.6028 µg/ml.
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