Anti-CrkII (phospho Tyr221) Antibody (A94393)

Name: Anti-CrkII (phospho Tyr221) Antibody
Brand: Antibodies.com
SKU: A94393
Price: 190.0 USD
Availability: InStock

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$190

Rabbit polyclonal antibody to CrkII (phospho Tyr221) for WB, IF and ELISA.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 6-8 business days.

Specifications

Name

Anti-CrkII (phospho Tyr221) Antibody

Description

Rabbit polyclonal antibody to CrkII (phospho Tyr221).

Specificity

This antibody detects endogenous levels of CrkII only when phosphorylated at Tyr221.

Applications

WB, IF, ELISA

Dilutions

WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:1000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human CrkII around the phosphorylation site of Tyr221 (amino acids 187-236).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

33kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Adapter molecule crk, p38, Proto-oncogene c-Crk

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-CrkII (phospho Tyr221) Antibody (A94393)

Western blot analysis of lysates from COS7 cells using Anti-CrkII (phospho Tyr221) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Immunofluorescence - Anti-CrkII (phospho Tyr221) Antibody (A94393)

Immunofluorescence analysis of HUVEC cells using Anti-CrkII (phospho Tyr221) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.