Western Blot - Anti-Crk p38 (phospho Tyr221) Antibody [ARC1640] (A306087)
Western blot analysis of extracts of various cell lines, using Anti-Crk p38 (phospho Tyr221) Antibody [ARC1640] (A306087) at 1:1,000 dilution. Both NIH/3T3 cells and C6 cells were treated by Calyculin A (100 nM) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 10s.
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