Anti-CRISPR-Cas9 Antibody [6F7] (A85319)

Transfected HEK293 cells with GFP-Cas9-SA fusion protein were stained with Anti-Cas9 from Staphylococcus Aureus Antibody. GFP is expressed in the transfected cells (green). GFP-Cas9 fusion protein is stained with Anti-Cas9 from Staphylococcus Aureus Antibody, at a dilution of 1:1,000, in red. Only transfected cells are reactive with Anti-Cas9 from Staphylococcus Aureus Antibody, which appear in a yellow-orange color (merge). Nuclei are visualized in blue with Hoechst staining.

Immunofluorescent analysis of HEK293 cells transfected with a construct of green fluorescent protein (GFP) fused with the C-terminal sequence (aa. 803-1053) of S. aureus Cas9. Cells were stained with Anti-Cas9 Antibody [6F7] (A85319) in red, and this combined with the expressed GFP gives a green signal. The two signals overlap as expected. The nuclei of both transfected and untransfected cells are revealed by the DAPI DNA stain.

Western blot analysis of HEK293 cell lysates using Anti-Cas9 Antibody [6F7] (A85319), in green. The lanes contain samples of: [Lane 1] Protein standards, in red, [Lane 2] non-transfected cells, and [Lane 3] cells transfected with a GFP-CAS9 fusion construct, encoding the C-terminal (803-1053) amino acids of S. aureus CAS9. The strong band at about 53 kDa corresponds to the GFP-CAS9 fusion protein.

Western blot analysis of Anti-Cas9 from Staphylococcus Aureus Antibody, at a dilution of 1:1,000). 1: HEK293 cells which overexpressed fusion protein containing GFP and c-terminus of Cas9 from S. aureus. 2: Non-transfected HEK293 cells. There is a strong clean band at about 53 kDa corresponding to GFP-Cas9 fusion protein, which is absent in non-transfected cells.