Anti-CRISPR-Cas9 Antibody [3F9] (A85327)

Transfected HEK293 cells overexpressing the N-terminal 1-608 amino acids of S. pyogenes Cas9 . The cells were stained with Anti-Cas9 from Streptococcus Pyogenes Antibody (red) and Anti-Cas9 from Streptococcus Pyogenes Antibody (A85326 | green) giving these cells a yellow appearance. The N-terminal construct contains a nuclear localization sequence and so is predominantly nuclear in localization. Most HEK293 cells in this field are not transfected so only the nuclei of these cells can be visualized with the blue DAPI DNA stain.

Western blot analysis of Anti-Cas9 from Streptococcus Pyogenes Antibody. Lane 1: Protein size marker with size in kiloDaltons. Lane 2: Blot of crude protein extract from HEK293 culture transfected with the Anti-Cas9 from Streptococcus Pyogenes Antibody immunogen, the N-terminal 1-608 amino acids. Lane 3: Non-transfected control HEK293 cell extract. Lane 4: Blot of 40 ng Streptococcus pyogenes Cas9 was probed with Anti-Cas9 from Streptococcus Pyogenes Antibody (1:1,000) and, as expected, the antibody also recognizes full length Cas9 protein at 160 kDa.