Anti-CREB (phospho Ser133) Antibody (A10868)

Name: Anti-CREB (phospho Ser133) Antibody
Brand: Antibodies.com
SKU: A10868
Price: 435.0 USD
Availability: InStock

Enlarge Image

$435

Rabbit polyclonal antibody to CREB (phospho Ser133) for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

Contact Us

Telephone

+1 (314) 370-6046

orders@antibodies.com

Guarantees

100% Quality Guarantee

Price Match Guarantee

Specifications

Name

Anti-CREB (phospho Ser133) Antibody

Description

Rabbit polyclonal antibody to CREB (phospho Ser133).

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:200-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:100

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic phosphorylated peptide around S133 of human CREB1 (NP_604391.1).

Sequence

RPSYR

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

43 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Active transcription factor CREB, cAMP response element binding protein, cAMP response element binding protein 1, cAMP responsive element binding protein 1, cAMP-responsive element-binding protein 1, CREB-1, CREB1, CREB1_HUMAN, Cyclic AMP-responsive element-binding protein 1, MGC9284, OTTHUMP00000163864, OTTHUMP00000163865, OTTHUMP00000206660, OTTHUMP00000206662, OTTHUMP00000206667, Transactivator protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

Enlarge Image

Figure 1: Western Blot - Anti-CREB (phospho Ser133) Antibody (A10868)

Western blot analysis of extracts of HeLa cells, using Anti-CREB (phospho Ser133) Antibody (A10868) at 1:2,000 dilution or CREB1 antibody (A11064). HeLa cells were treated by Anisomycin (25 ug/mL) at 37°C for 30 minutes after serum-starvation overnight. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% BSA. Detection was with a ECL Basic Kit. Exposure time: 30s.

Enlarge Image

Figure 2: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10868)

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-CREB (phospho Ser133) Antibody (A10868) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 3: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10868)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-CREB (phospho Ser133) Antibody (A10868) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Figure 4: Immunohistochemistry - Anti-CREB (phospho Ser133) Antibody (A10868)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-CREB (phospho Ser133) Antibody (A10868) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.