Figure 1: Western Blot - Anti-Creatine Kinase MM Antibody (A13818)
Western blot analysis of extracts of Mouse skeletal muscle, using Anti-Creatine Kinase MM Antibody (A13818) at 1:3,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Figure 2: Immunofluorescence - Anti-Creatine Kinase MM Antibody (A13818)
Immunofluorescence analysis of mouse heart cells using Anti-Creatine Kinase MM Antibody (A13818) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
Figure 3: Immunofluorescence - Anti-Creatine Kinase MM Antibody (A13818)
Immunofluorescence analysis of rat heart cells using Anti-Creatine Kinase MM Antibody (A13818) at a dilution of 1:20 (40x lens). DAPI was used to stain the cell nuclei (blue). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.
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