Mouse monoclonal [CKBB/6567] antibody to Creatine Kinase MB.
Specificity
Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.
Applications
ELISA, WB, IHC-P
Positive Control
SH-SY5Y or HEK-293 cells. Human cerebellum.
Dilutions
WB: 1-2 µg/ml, IHC: 1-2 µg/ml
Reactivity
Human
Immunogen
Recombinant human full-length CKB protein.
Host
Mouse
Clonality
Monoclonal
Clone ID
CKBB/6567
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
42 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.