Anti-CPT1A Antibody (A14655)

Name: Anti-CPT1A Antibody
Brand: Antibodies.com
SKU: A14655
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to CPT1A for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-CPT1A Antibody

Description

Rabbit polyclonal antibody to CPT1A.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 520-720 of human CPT1A (NP_001867.2).

Sequence

WDIPGECQEVIETSLNTANLLANDVDFHSFPFVAFGKGIIKKCRTSPDAFVQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTTESCDFVRAMVDPAQTVEQRLKLFKLASEKHQHMYRLAMTGSGIDRHLFCLYVVSKYLAVESPFLKEVLSEPWRLSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

88 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Carnitine O palmitoyltransferase 1 liver isoform, Carnitine O palmitoyltransferase I, Carnitine O palmitoyltransferase I liver isoform, Carnitine O-palmitoyltransferase 1, Carnitine O-palmitoyltransferase I, Carnitine palmitoyltransferase 1A, Carnitine palmitoyltransferase 1A (liver), Carnitine palmitoyltransferase I, Carnitine palmitoyltransferase I liver, CPT 1, CPT I, CPT1, CPT1 L, CPT1-L, CPT1A_HUMAN, CPTI, CPTI-L, L CPT1, liver isoform

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-CPT1A Antibody (A14655)

Western blot analysis of Mouse heart, using Anti-CPT1A Antibody (A14655) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-CPT1A Antibody (A14655)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CPT1A Antibody (A14655) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-CPT1A Antibody (A14655)

Immunofluorescence analysis of HeLa cells using Anti-CPT1A Antibody (A14655) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-CPT1A Antibody (A14655)

Immunoprecipitation analysis of 300µg extracts of MCF7 cells using 3µg of Anti-CPT1A Antibody (A14655). This Western blot was performed on the immunoprecipitate using Anti-CPT1A Antibody (A14655) at a dilution of 1:1000.

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