Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
COX IV-1, COX4, COX4I1, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome c oxidase subunit IV isoform 1
Western Blot - Anti-COX IV Antibody [ARC2518] (A81073)
Western blot analysis of extracts of various cell lines, using Anti-COX IV Antibody [ARC2518] (A81073) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry - Anti-COX IV Antibody [ARC2518] (A81073)
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using Anti-COX IV Antibody [ARC2518] (A81073) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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