Anti-COX IV Antibody (A304966)

Name: Anti-COX IV Antibody
Brand: Antibodies.com
SKU: A304966
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to COX IV for WB and ICC/IF.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Mitochondrial Loading Control Mitochondrial Marker

Specifications

Name

Anti-COX IV Antibody

Description

Rabbit polyclonal antibody to COX IV.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide of Human COX-4.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AL024441, COX 4, COX IV 1, COX IV-1, Cox4, COX4-1, COX41_HUMAN, Cox4a, COX4B, COX4I1, COX4I2, COX4L2, COXIV, Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit 4 isoform 1, Cytochrome c oxidase subunit 4 isoform 1 mitochondrial, Cytochrome c oxidase subunit 4 isoform 1, mitochondrial, Cytochrome C Oxidase subunit IV, Cytochrome c oxidase subunit IV isoform 1, Cytochrome c oxidase subunit IV isoform 2 (lung), Cytochrome c oxydase subunit 4, dJ857M17.2, MGC105470, MGC72016, mitochondrial

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-COX IV Antibody (A304966)

Western blot analysis of human HeLa and HEK293T cell lysates showing detection of 19.5 kDa COX-4 protein using Anti-COX IV Antibody (A304966) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa cell lysates. Lane 3: 293Trap cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 19.5 kDa.

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Figure 2: Immunofluorescence - Anti-COX IV Antibody (A304966)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-COX IV Antibody (A304966), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm, Mitochondrion. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) COX-4 Antibody (D) Composite.

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Figure 3: Immunofluorescence - Anti-COX IV Antibody (A304966)

Immunocytochemistry/Immunofluorescence analysis of mouse NIH 3T3 (mouse fibroblast cell line), fixed in 4% formaldehyde for 15 min at room temperature, using Anti-COX IV Antibody (A304966), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Mitochondrion Inner Membrane. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) COX-4 Antibody (D) Composite.

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