Anti-Cortactin Antibody [ARC1613] (A307999)

Name: Anti-Cortactin Antibody [ARC1613]
Brand: Antibodies.com
SKU: A307999
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1613] antibody to Cortactin for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Cortactin Antibody [ARC1613]

Description

Rabbit monoclonal [ARC1613] antibody to Cortactin.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Cortactin (Q14247).

Sequence

MWKASAGHAVSIAQDDAGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDKSAV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1613

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 - 85 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Amplaxin, CTTN, EMS 1, EMS1, FLJ34459, Mammary tumor and squamous cell carcinoma associated, Oncogene EMS1, p80/85 src substrate, Src substrate cortactin, SRC8_HUMAN

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-Cortactin Antibody [ARC1613] (A307999)

Western blot analysis of extracts of various cell lines, using Anti-Cortactin Antibody [ARC1613] (A307999) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 3s.

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Figure 2: Immunohistochemistry - Anti-Cortactin Antibody [ARC1613] (A307999)

Immunohistochemistry analysis of paraffin-embedded rat testis using Anti-Cortactin Antibody [ARC1613] (A307999) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Cortactin Antibody [ARC1613] (A307999)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Cortactin Antibody [ARC1613] (A307999) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Cortactin Antibody [ARC1613] (A307999)

Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-Cortactin Antibody [ARC1613] (A307999) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.