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Anti-COP Antibody (A285928)

Immunofluorescence - Anti-COP Antibody (A285928) - Antibodies.com Flow Cytometry - Anti-COP Antibody (A285928) - Antibodies.com
$480
Product Datasheet

Goat polyclonal antibody to COP for ELISA, IF and Flow Cytometry.

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Freight Charges
$40 to United States
Lead Time
3-5 Business Days

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Name
Anti-COP Antibody
Description
Goat polyclonal antibody to COP.
Applications
ELISA, IF, Flow Cytometry
Dilutions
ELISA: 1:4,000, IF: 10µg/ml, Flow Cytometry: 10µg/ml
Reactivity
Human
Cross Reactivity
This antibody is expected to cross react with INCA (GeneID 440068; NP_001007233.1).
Immunogen
Synthetic peptide corresponding to the C terminal region of human COP.
Sequence
C-ETLGLSAGPIPGN
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
CAR16_HUMAN, CARD only domain-containing protein 1, CARD only protein, CARD16, Caspase recruitment domain family, member 16, Caspase recruitment domain-containing protein 16, Caspase-1 dominant-negative inhibitor pseudo-ICE, Caspase-1 inhibitor COP, COP1, Pseudo interleukin 1 beta converting enzyme, Pseudo interleukin-1 beta converting enzyme, Pseudo interleukin-1beta converting enzyme, PSEUDO-ICE
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Immunofluorescence - Anti-COP Antibody (A285928) - Antibodies.comEnlarge Image
Figure 1: Immunofluorescence - Anti-COP Antibody (A285928)
COP expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-COP Antibody (A285928) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Mitochondrial staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.
Flow Cytometry - Anti-COP Antibody (A285928) - Antibodies.comEnlarge Image
Figure 2: Flow Cytometry - Anti-COP Antibody (A285928)
COP expression in A549 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-COP Antibody (A285928) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.
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