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Anti-Collagen XVII Antibody [ARC0233] (A308839)

Western Blot - Anti-Collagen XVII Antibody [ARC0233] (A308839) - Antibodies.com Immunohistochemistry - Anti-Collagen XVII Antibody [ARC0233] (A308839) - Antibodies.com
$470
Product Datasheet

Rabbit monoclonal [ARC0233] antibody to Collagen XVII for WB and IHC.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Collagen XVII Antibody [ARC0233]
Description
Rabbit monoclonal [ARC0233] antibody to Collagen XVII.
Applications
WB, IHC
Dilutions
WB: 1:500-1:2,000, IHC: 1:50-1:200
Reactivity
Human
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of human Collagen XVII/COL17A1 (Q9UMD9).
Sequence
SVRRGSSYSSSMSTGGGGAGSLGAGGAFGEAAGDRGPYGTDIGPGGGYGAAAEGGMYAGNGGLLGADFAGDLDYNELAVRVSESMQRQGLLQGMAYTVQGP
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC0233
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
180 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
180 kDa bullous pemphigoid antigen 2, Alpha 1 type XVII collagen, BA16H23.2, BP 180, BP180, BPA 2, BPAG 2, BPAG2, Bullous pemphigoid antigen 2, COL17A1, Collagen 17, Collagen alpha 1 XVII chain, Collagen alpha 1(XVII) chain, Collagen alpha1 XVII chain, Collagen type XVII alpha 1, Collagen XVII alpha 1 polypeptide, CollagenXVII, Epidermolysis bullosa junctional localisata variant, FLJ60881, KIAA0204, LAD 1, LAD1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Collagen XVII Antibody [ARC0233] (A308839) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Collagen XVII Antibody [ARC0233] (A308839)
Western blot analysis of extracts of A-431 cells, using Anti-Collagen XVII Antibody [ARC0233] (A308839) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-Collagen XVII Antibody [ARC0233] (A308839) - Antibodies.comEnlarge Image
Figure 2: Immunohistochemistry - Anti-Collagen XVII Antibody [ARC0233] (A308839)
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Collagen XVII Antibody [ARC0233] (A308839) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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