Anti-Collagen XVII Antibody [ARC0233] (A308839)

Name: Anti-Collagen XVII Antibody [ARC0233]
Brand: Antibodies.com
SKU: A308839
Price: 485.0 USD
Availability: InStock

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$485

Rabbit monoclonal [ARC0233] antibody to Collagen XVII for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-Collagen XVII Antibody [ARC0233]

Description

Rabbit monoclonal [ARC0233] antibody to Collagen XVII.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of human Collagen XVII/COL17A1 (Q9UMD9).

Sequence

SVRRGSSYSSSMSTGGGGAGSLGAGGAFGEAAGDRGPYGTDIGPGGGYGAAAEGGMYAGNGGLLGADFAGDLDYNELAVRVSESMQRQGLLQGMAYTVQGP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0233

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

180 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

180 kDa bullous pemphigoid antigen 2, BP180, BPAG2, Bullous pemphigoid antigen 2, COL17A1, Collagen alpha-1(XVII) chain

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (2)

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Western Blot - Anti-Collagen XVII Antibody [ARC0233] (A308839)

Western blot analysis of extracts of A-431 cells, using Anti-Collagen XVII Antibody [ARC0233] (A308839) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-Collagen XVII Antibody [ARC0233] (A308839)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-Collagen XVII Antibody [ARC0233] (A308839) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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