Anti-Collagen VI Antibody [ARC1725] (A305599)

Name: Anti-Collagen VI Antibody [ARC1725]
Brand: Antibodies.com
SKU: A305599
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC1725] antibody to Collagen VI for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-Collagen VI Antibody [ARC1725]

Description

Rabbit monoclonal [ARC1725] antibody to Collagen VI.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 37-235 of human Collagen VI/COL6A1 (P12109).

Sequence

DLFFVLDTSESVALRLKPYGALVDKVKSFTKRFIDNLRDRYYRCDRNLVWNAGALHYSDEVEIIQGLTRMPGGRDALKSSVDAVKYFGKGTYTDCAIKKGLEQLLVGGSHLKENKYLIVVTDGHPLEGYKEPCGGLEDAVNEAKHLGVKVFSVAITPDHLEPRLSIIATDHTYRRNFTAADWGQSRDAEEAISQTIDTI

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC1725

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

108 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Alpha 1 (VI) chain (61 AA), CO6A1_HUMAN, COL6A1, COL6A2, COL6A3, Collagen alpha 2(VI) chain, Collagen alpha 3(VI) chain, Collagen alpha-1(VI) chain, Collagen type VI alpha 1, Collagen type VI alpha 2, Collagen type VI alpha 3, Collagen VI alpha 1 polypeptide, Collagen VI alpha 2 polypeptide, Collagen VI alpha 3 polypeptide, CollagenVI, Human mRNA for collagen VI alpha 2 C terminal globular domain, OPLL, PP3610

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-Collagen VI Antibody [ARC1725] (A305599)

Western blot analysis of extracts of various cell lines, using Anti-Collagen VI Antibody [ARC1725] (A305599) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.

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Figure 2: Immunohistochemistry - Anti-Collagen VI Antibody [ARC1725] (A305599)

Immunohistochemistry analysis of paraffin-embedded rat lung using Anti-Collagen VI Antibody [ARC1725] (A305599) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-Collagen VI Antibody [ARC1725] (A305599)

Immunohistochemistry analysis of paraffin-embedded human liver using Anti-Collagen VI Antibody [ARC1725] (A305599) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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