Anti-Collagen IV Antibody (A282757)

Anti-Collagen IV Antibody

Rabbit polyclonal antibody to Collagen IV.

This antibody recognises mouse collagen type IV. Collagen IV is a 1682 amino acid ~160 kDa (predicted) matrx protein and major component of glomerular basement membranes. Multiple isoforms exist each capable of forming triple helical structures with two other chains to form the type IV collagen network. The collagen IV alpha chain can be cleaved between residues 1444-1445 to yield the C-terminal 225 amino acid, ~28 kDa arresten fragment, collagen a2(IV) yields a C-terminal canstatin fragment while Collagen a3(IV) yeilds a tumstatin fragment. Collagen IV bears a single collagen IV NC1 (C-terminal non-collagenous) domain (UniProt: Q9QZR9).Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms. Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice , rats and oragutan. The following cross reactivities have been observed:Mouse type IV100% Mouse types I, II & III<0.1% Human types IV & V<0.1% Mouse fibronectin & laminin<0.1%

IHC-P, ELISA, IF, IHC-Fr

ELISA: 1:2,000, IF: 1:40, IHC-Fr: 1:500, IHC-P: 1:500

Mouse, Orangutan, Rat, Human

Collagen IV purified from mouse EHS tumor.

Rabbit

Polyclonal

IgG

Lot Specific

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide and Antibiotic antimycotic mixture 1%.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rabbit anti Mouse Collagen IV antibody recognizes mouse collagen type IV. Collagen IV is a 1682 amino acid ~160 kDa (predicted) matrx protein and major component of glomerular basement membranes. Multiple isoforms exist each capable of forming triple helical structures with two other chains to form the type IV collagen network. The collagen IV alpha chain can be cleaved between residues 1444-1445 to yield the c-terminal 225 amino acid, ~28 kDa arresten fragment, collagen a2(IV) yields a c-terminal canstatin fragment while Collagen a3(IV) yeilds a tumstatin fragment. Collagen IV bears a single collagen IV NC1 (C-terminal non-collagenous) domain (UniProt: Q9QZR9).Mutations in collagen IV genes have been implicated in inherited nephropathies and potentially in cystic kidney disease and intracranial aneurysms (Plaisier et al. 2007).Rabbit amti Mouse Collagen IV antibody has been successfully employed for the detection of collagen IV by immunofluorescence and immunohistochemistry in mice (Tang et al. 2010), rats (Shen et al. 2014) and oragutan (Bredies et al. 2013).The following cross reactivities have been observed:Mouse type IV100%Mouse types I, II & III<0.1%Human types IV & V<0.1%Mouse fibronectin & laminin<0.1%

COL4A4, Collagen alpha-4(IV) chain