Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Alpha 1 type II collagen, Alpha-1 type II collagen, AOM, Cartilage collagen, Chondrocalcin, CO2A1_HUMAN, COL11A3, Col2a1, Collagen II alpha 1 polypeptide, SEDC
Figure 1: Western Blot - Anti-Collagen II Antibody (A306085)
Western blot analysis of extracts of Mouse skeletal muscle, using Anti-Collagen II Antibody (A306085) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Figure 2: Immunofluorescence - Anti-Collagen II Antibody (A306085)
Immunofluorescence analysis of rat trachea using Anti-Collagen II Antibody (A306085) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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