Western blot analysis of extracts of various cell lines, using Anti-Collagen I Antibody (A88196) at 1:2,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunohistochemistry - Anti-Collagen I Antibody (A88196)
Immunohistochemistry analysis of paraffin-embedded human breast cancer tissue using Anti-Collagen I Antibody (A88196) at a dilution of 1:10000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Collagen I Antibody (A88196)
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Collagen I Antibody (A88196) at a dilution of 1:10000 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-Collagen I Antibody (A88196)
Immunofluorescence analysis of NIH/3T3 cells using Anti-Collagen I Antibody (A88196) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-Collagen I Antibody (A88196)? Please let us know so that we can list the citation on this page.
Alternative products to Anti-Collagen I Antibody (A88196)
