Immunofluorescent analysis of a section of mouse striatum stained using Anti-CNPase Antibody (A270543), at a dilution of 1:3,000, in green, and co-stained with Anti-NF-L Antibody (A85451), at a dilution of 1:5,000, in red. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion of the mouse with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained with the above antibodies. The Anti-CNPase Antibody (A270543) stains myelin sheath and the plasma membranes of oligodendrocytes, the myelin producing cells of the CNS. The Anti-NF-L Antibody (A85451) labels axons of neuronal cells enclosed by the myelin.
Figure 2: Western Blot - Anti-CNPase Antibody (A270543)
Western blot analysis of different tissue lysates using Anti-CNPase Antibody (A270543), at a dilution of 1:5,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46 kDa and 48 kDa correspond to the two major isotypes of CNP protein.
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