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CNOT2 Cell Based ELISA Kit (A103082)

Western Blot - CNOT2 Cell Based ELISA Kit (CB5150) - Antibodies.com Protocol Diagram - CNOT2 Cell Based ELISA Kit (CB5150) - Antibodies.com Protocol Summary - CNOT2 Cell Based ELISA Kit (CB5150) - Antibodies.com Western Blot - Control Antibody - CNOT2 Cell Based ELISA Kit (CB5150) - Antibodies.com
$550
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$40 to United States
Lead Time
5-8 Business Days

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Name
CNOT2 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This CNOT2 Cell Based ELISA Kit allows for the detection of CNOT2 and the effects that certain stimulation conditions have on CNOT2 expression in different cell lines. Qualitative determination of CNOT2 concentration is achieved by an indirect ELISA format. In essence, the CNOT2 is captured by Anti-CNOT2 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CNOT2 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
CCR4 associated factor 2, CCR4 NOT transcription complex subunit 2, CCR4-associated factor 2, CCR4-NOT transcription complex subunit 2, CDC36, CNOT2_HUMAN, HSPC131, MSTP046, Negative regulator of transcription 2, NOT2, NOT2 (negative regulator of transcription 2 yeast) homolog, NOT2H
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - CNOT2 Cell Based ELISA Kit (CB5150) - Antibodies.comEnlarge Image
Figure 1: Western Blot - CNOT2 Cell Based ELISA Kit (A103082)
Western blot analysis of extracts from HepG2 cells, treated with starved 24h, using Anti-CNOT2 (Ab-101) Antibody.
Protocol Diagram - CNOT2 Cell Based ELISA Kit (CB5150) - Antibodies.comEnlarge Image
Figure 2: CNOT2 Cell Based ELISA Kit (A103082)
A diagram showing the protocol for CNOT2 Cell Based ELISA Kit.
Protocol Summary - CNOT2 Cell Based ELISA Kit (CB5150) - Antibodies.comEnlarge Image
Figure 3: CNOT2 Cell Based ELISA Kit (A103082)
A summary of the protocol for CNOT2 Cell Based ELISA Kit.
Western Blot - Control Antibody - CNOT2 Cell Based ELISA Kit (CB5150) - Antibodies.comEnlarge Image
Figure 4: Western Blot - CNOT2 Cell Based ELISA Kit (A103082)
The mouse monoclonal antibody to GAPDH is used as a positive control inCNOT2 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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