Thyroid Peroxidase/TPO expression in Human Thyroid Gland analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-Thyroid Peroxidase/TPO Antibody (A83130) at 5µg/ml and revealed with alkaline phosphatase (AP).
Western Blot - Anti-Thyroid Peroxidase/TPO Antibody (A83130)
Thyroid Peroxidase/TPO expression in Thyroid Gland lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed for 1 hour with Anti-Thyroid Peroxidase/TPO Antibody (A83130) at 0.1µg/ml and detected by chemiluminescence.
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