HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human STAT4 (RC206892) with C-terminal MYC tag probed with Anti-STAT4 Antibody (A84780) (1µg/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with Anti-STAT4 Antibody (A84780) (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
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