Anti-STAT3 Antibody (A13365)

Name: Anti-STAT3 Antibody
Brand: Antibodies.com
SKU: A13365
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to STAT3 for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-STAT3 Antibody

Description

Rabbit polyclonal antibody to STAT3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 600-700 of human STAT3 (NP_003141.2).

Sequence

TKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

86 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Acute-phase response factor, APRF, Signal transducer and activator of transcription 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (11)

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Western Blot - Anti-STAT3 Antibody (A13365)

Western blot analysis of various lysates, using Anti-STAT3 Antibody (A13365) at 1:2,500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.

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Immunohistochemistry - Anti-STAT3 Antibody (A13365)

Immunohistochemistry analysis of paraffin-embedded mouse brain using Anti-STAT3 Antibody (A13365) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-STAT3 Antibody (A13365)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-STAT3 Antibody (A13365) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-STAT3 Antibody (A13365)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-STAT3 Antibody (A13365) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.