Anti-SOCS3 Antibody (A12687)

Name: Anti-SOCS3 Antibody
Brand: Antibodies.com
SKU: A12687
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to SOCS3 for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-SOCS3 Antibody

Description

Rabbit polyclonal antibody to SOCS3.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human SOCS3 (NP_003946.3).

Sequence

GSFSLQSDPRSTQPVPRFDCVLKLVHHYMPPPGAPSFPSPPTEPSSEVPEQPSAQPLPGSPPRRAYYIYSGGEKIPLVLSRPLSSNVATLQHLCRKTVNGHLDSYEKVTQLPGPIREFLDQYDAPL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

30 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ATOD4, CIS 3, CIS-3, CIS3, Cish3, Cytokine induced SH2 protein 3, Cytokine-inducible SH2 protein 3, E2a Pbx1 target gene in fibroblasts 10, EF 10, MGC71791, SOCS 3, SOCS-3, SOCS3_HUMAN, SSI 3, SSI-3, SSI3, STAT induced STAT inhibitor 3, STAT-induced STAT inhibitor 3, Suppressor of cytokine signaling 3

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-SOCS3 Antibody (A12687)

Western blot analysis of extracts of RAW264. 7 cells, using Anti-SOCS3 Antibody (A12687) at 1:500 dilution. Raw264. 7 cells were treated by LPS (1 µg/ml) at 37°C for 8 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Immunohistochemistry - Anti-SOCS3 Antibody (A12687)

Immunohistochemistry analysis of paraffin-embedded mouse heart using Anti-SOCS3 Antibody (A12687) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-SOCS3 Antibody (A12687)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-SOCS3 Antibody (A12687) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-SOCS3 Antibody (A12687)

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-SOCS3 Antibody (A12687) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.