Anti-IL-18 Antibody (A83377)

Name: Anti-IL-18 Antibody
Brand: Antibodies.com
SKU: A83377
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to IL-18 for ELISA, WB, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-IL-18 Antibody

Description

Goat polyclonal antibody to IL-18.

Applications

ELISA, WB, IF, Flow Cytometry

Dilutions

ELISA: 1:32,000, WB: 1-3µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human IL-18.

Sequence

C-NPPDNIKDTKSDI

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

23 kDa

Predicted MW

22.3kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Iboctadekin, IFN gamma inducing factor, IFN-gamma-inducing factor, IGIF, IL 1 gamma, IL 18, IL 1g, IL-1 gamma, IL1 gamma, IL18, IL18 protein, IL18_HUMAN, IL1F4, IL1g, IL1gamma, ILIF4, Interferon gamma inducing factor, Interferon gamma-inducing factor, Interleukin 1 gamma, Interleukin 18, Interleukin 18 (interferon-gamma-inducing factor), Interleukin-1 gamma, Interleukin-18, Interleukin18, MGC12320

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-IL-18 Antibody (A83377)

IL-18 expression in HeLa (A), A431 (B), and US0S (C) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-IL-18 Antibody (A83377) at 0.1µg/ml (A) or 2µg/ml (B-C) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-IL-18 Antibody (A83377)

IL-18 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-IL-18 Antibody (A83377) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and Golgi apparatus staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Immunofluorescence - Anti-IL-18 Antibody (A83377)

IL-18 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-IL-18 Antibody (A83377) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic, Golgi apparatus, and nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Flow Cytometry - Anti-IL-18 Antibody (A83377)

IL-18 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-IL-18 Antibody (A83377) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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