Anti-GRP78 BiP Antibody (A12567)

Name: Anti-GRP78 BiP Antibody
Brand: Antibodies.com
SKU: A12567
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to GRP78 BiP for WB and IHC.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-GRP78 BiP Antibody

Description

Rabbit polyclonal antibody to GRP78 BiP.

Applications

WB, IHC

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 550-650 of human BiP/GRP78 (NP_005338.1).

Sequence

EEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAE

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

78 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

78 kDa glucose-regulated protein, Binding-immunoglobulin protein, Endoplasmic reticulum chaperone BiP, GRP-78, GRP78, Heat shock protein 70 family protein 5, Heat shock protein family A member 5, HSP70 family protein 5, HSPA5, Immunoglobulin heavy chain-binding protein

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-GRP78 BiP Antibody (A12567)

Western blot analysis of extracts of various cell lines, using Anti-GRP78 BiP Antibody (A12567) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunohistochemistry - Anti-GRP78 BiP Antibody (A12567)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-GRP78 BiP Antibody (A12567) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-GRP78 BiP Antibody (A12567)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-GRP78 BiP Antibody (A12567) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-GRP78 BiP Antibody (A12567)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-GRP78 BiP Antibody (A12567) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.