Anti-HIF-2 alpha Antibody (A10163)

Name: Anti-HIF-2 alpha Antibody
Brand: Antibodies.com
SKU: A10163
Price: 345.0 USD
Availability: InStock

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$345

Rabbit polyclonal antibody to HIF-2 alpha for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-HIF-2 alpha Antibody

Description

Rabbit polyclonal antibody to HIF-2 alpha.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 678-745 of human EPAS1/HIF2a (NP_001421.2).

Sequence

TFKTRSAKGFGARGPDVLSPAMVALSNKLKLKRQLEYEEQAFQDLSGGDPPGGSTSHLMWKRMKNLRG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Basic-helix-loop-helix-PAS protein MOP2, BHLHE73, Class E basic helix-loop-helix protein 73, Endothelial PAS domain-containing protein 1, EPAS-1, EPAS1, HIF-1-alpha-like factor, HIF2-alpha, HIF2A, HLF, Hypoxia-inducible factor 2-alpha, Member of PAS protein 2, MOP2, PAS domain-containing protein 2, PASD2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-HIF-2 alpha Antibody (A10163)

Western blot analysis of extracts of various cell lines, using Anti-HIF-2-alpha Antibody (A10163) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-HIF-2 alpha Antibody (A10163)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-HIF-2-alpha Antibody (A10163) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-HIF-2 alpha Antibody (A10163)

Immunohistochemistry analysis of paraffin-embedded human brain tissue using Anti-HIF-2-alpha Antibody (A10163) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.