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Anti-HDAC4 Antibody (A12565)

Western Blot - Anti-HDAC4 Antibody (A0239) - Antibodies.com Immunofluorescence - Anti-HDAC4 Antibody (A0239) - Antibodies.com Immunoprecipitation - Anti-HDAC4 Antibody (A0239) - Antibodies.com
This product is discontinued

Anti-HDAC4 Antibody (A12565) has been discontinued and is no longer available.

View all Anti-HDAC4 Antibodies.

Name
Anti-HDAC4 Antibody
Description
Rabbit polyclonal antibody to HDAC4.
Applications
WB, IF, IP
Dilutions
WB: 1:500-1:2000, IF: 1:50-1:200, IP: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide of human HDAC4.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
117kDa
Formulation
Supplied in Phosphate Buffered Saline, pH 7.30, with 0.02% Sodium Azide and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
AHO3, BDMR, EC 3.5.1.98, HA6116, HD 4, HD4, HDAC 4, HDAC A, HDAC4_HUMAN, HDACA, Histone deacetylase 4, Histone Deacetylase A, KIAA0288
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-HDAC4 Antibody (A0239) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-HDAC4 Antibody (A12565)
Western blot analysis of extracts of various cell lines, using Anti-HDAC4 Antibody (A0239) at 1:1,000 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L) (HRP) (AS014) at 1:10,000 dilution. Lysates / proteins: 25µg per lane. Blocking buffer: 3% non-fat dry milk in TBST.
Immunofluorescence - Anti-HDAC4 Antibody (A0239) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-HDAC4 Antibody (A12565)
Immunofluorescence analysis of A549 cells using Anti-HDAC4 Antibody (A0239). Blue: DAPI for nuclear staining.
Immunoprecipitation - Anti-HDAC4 Antibody (A0239) - Antibodies.comEnlarge Image
Figure 3: Immunoprecipitation - Anti-HDAC4 Antibody (A12565)
Immunoprecipitation analysis of 200µg extracts of HeLa cells, using 3µg Anti-HDAC4 Antibody (A0239). Western blot was performed from the immunoprecipitate using Anti-HDAC4 Antibody (A0239) at a dilition of 1:1000.
Publishing research using Anti-HDAC4 Antibody (A12565)? Please let us know so that we can list the citation on this page.

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