Anti-CPT1A Antibody (A14655)

Name: Anti-CPT1A Antibody
Brand: Antibodies.com
SKU: A14655
Price: 355.0 USD
Availability: InStock

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$355

Rabbit polyclonal antibody to CPT1A for WB, IHC, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Specifications

Name

Anti-CPT1A Antibody

Description

Rabbit polyclonal antibody to CPT1A.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 520-720 of human CPT1A (NP_001867.2).

Sequence

WDIPGECQEVIETSLNTANLLANDVDFHSFPFVAFGKGIIKKCRTSPDAFVQLALQLAHYKDMGKFCLTYEASMTRLFREGRTETVRSCTTESCDFVRAMVDPAQTVEQRLKLFKLASEKHQHMYRLAMTGSGIDRHLFCLYVVSKYLAVESPFLKEVLSEPWRLSTSQTPQQQVELFDLENNPEYVSSGGGFGPVADDGY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

88 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Carnitine O-palmitoyltransferase 1, liver isoform, Carnitine O-palmitoyltransferase I, liver isoform, Carnitine palmitoyltransferase 1A, CPT I, CPT1, CPT1-L, CPTI-L, Succinyltransferase CPT1A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-CPT1A Antibody (A14655)

Western blot analysis of Mouse heart, using Anti-CPT1A Antibody (A14655) at 1:400 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Immunohistochemistry - Anti-CPT1A Antibody (A14655)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CPT1A Antibody (A14655) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-CPT1A Antibody (A14655)

Immunofluorescence analysis of HeLa cells using Anti-CPT1A Antibody (A14655) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Western Blot - Anti-CPT1A Antibody (A14655)

Immunoprecipitation analysis of 300µg extracts of MCF7 cells using 3µg of Anti-CPT1A Antibody (A14655). This Western blot was performed on the immunoprecipitate using Anti-CPT1A Antibody (A14655) at a dilution of 1:1000.

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