Anti-Clostridium difficile Toxin A Antibody [253/422] - BSA and Azide free (A254034)

Mouse monoclonal [253/422] antibody to Clostridium difficile Toxin A for ELISA and IF.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
4-6 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Anti-Clostridium difficile Toxin A Antibody [253/422] - BSA and Azide free
Description
Mouse monoclonal [253/422] antibody to Clostridium difficile Toxin A.
Specificity
This antibody reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.
Applications
ELISA, IF
Dilutions
ELISA: 1-5 µg/ml, IF: 1-2 µg/ml
Reactivity
Clostridium difficile
Immunogen
C. difficile Toxin A.
Host
Mouse
Clonality
Monoclonal
Clone ID
253/422
Isotype
IgG1
Light Chains
kappa
Conjugate

Unconjugated

Purification
Protein A/G chromatography.
Concentration
1 mg/ml
Molecular Weight
308 kDa (Toxin A)
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
General Notes
This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-Clostridium difficile Toxin A Antibody [253/422] (A250854).
Synonyms
tcdA, toxA
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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