This antibody recognizes a protein of 27kDa, identified as CLEC9A (C-type lectin domain family 9 member A). Its epitopes maps in aa 50-110. The C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily consists of a variety of proteins that share a common protein fold and have diverse functions, including cell-cell signaling, cell adhesion, glycoprotein turnover and immune responses. CLEC-9A, also known as DNGR1 (dendritic cell natural killer lectin group receptor 1), is a 241 amino acid single-pass type II membrane protein that contains one C-type lectin domain and belongs to the CTL/CTLD superfamily. Expressed in myeloid lineage cells, brain, spleen and thymus, CLEC-9A is a group V C-type lectin- like receptor (CTLR) that acts as an activation receptor.

Applications

ELISA, Flow Cytometry, IF

Dilutions

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Reactivity

Human

Immunogen

RBL-2H3 cells expressing human DNGR-1 fused to an HA epitope.

Host

Mouse

Clonality

Monoclonal

Clone ID

8F9

Isotype

IgG2a

Light Chains

kappa

Conjugate

Unconjugated

Purification

Protein A/G chromatography.

Concentration

1 mg/ml

Molecular Weight

27 kDa

Product Form

Liquid

Formulation

Supplied in 10mM Phosphate Buffered Saline; without Sodium Azide and carrier free.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

General Notes

This monoclonal antibody is also available in a different formulation with BSA and Sodium Azide - Anti-CLEC9A Antibody [8F9] (A248754).

Synonyms

C type lectin domain family 9, member A, C-type lectin domain family 9 member A, CD370, CLC9A_HUMAN, DNGR1, HEEE9341, PRO34046, UNQ9341

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (1)

Enlarge Image

Figure 1: SDS-PAGE - Anti-CLEC9A Antibody [8F9] - BSA and Azide free (A251934)

SDS-PAGE analysis of Anti-CLEC9A Antibody [8F9] under non-reduced and reduced conditions; showing intact IgG and intact heavy and light chains, respectively. SDS-PAGE analysis confirms the integrity and purity of the antibody.

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