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Anti-Cleaved GSDMD Antibody [ARC57994] (A306734)

Western Blot - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) - Antibodies.com Immunohistochemistry - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) - Antibodies.com Immunohistochemistry - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) - Antibodies.com Immunohistochemistry - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) - Antibodies.com Immunohistochemistry - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) - Antibodies.com
This product is discontinued

Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) has been discontinued and is no longer available.

View all available products.

  • Specifications

Name
Anti-Cleaved GSDMD Antibody [ARC57994]
Description
Rabbit monoclonal [ARC57994] antibody to Cleaved GSDMD.
Applications
WB, IHC
Dilutions
WB: 1:500-1:1,000, IHC: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Cleaved GSDMD (N Teminal) (NP_079012.3).
Sequence
LSQKKTVTIPSGSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPATGHKRSTSEGAWPQLPSGLSMMRCLHNFLTDGVPAEGAFTEDFQGLRAEVETISKE
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC57994
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
30 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (5)

Western Blot - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) - Antibodies.comEnlarge Image

Western Blot - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734)

Western blot analysis of various lysates, using Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) at 1:800 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37°C for overnight and LPS (1 µg/ml) at 37°C for 6 hours. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734)

Immunohistochemistry analysis of paraffin-embedded human stomach using Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) at dilution of1:1000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) at dilution of1:1000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-Cleaved GSDMD Antibody [ARC57994] (A306734)

Immunohistochemistry analysis of paraffin-embedded mouse stomach using Anti-Cleaved GSDMD Antibody [ARC57994] (A306734) at dilution of1:1000(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.