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CKS1 Cell Based ELISA Kit (A103545)

Western Blot - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.com Protocol Diagram - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.com Protocol Summary - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.com Western Blot - Control Antibody - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.com
$550
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$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
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Name
CKS1 Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This CKS1 Cell Based ELISA Kit allows for the detection of CKS1 and the effects that certain stimulation conditions have on CKS1 expression in different cell lines. Qualitative determination of CKS1 concentration is achieved by an indirect ELISA format. In essence, the CKS1 is captured by Anti-CKS1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this CKS1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
CDC2 associated protein CKS1, CDC28 protein kinase 1, CDC28 protein kinase 1B, CDC28 protein kinase regulatory subunit 1B, Cell division control protein CKS1, CKS 1, CKS-1, Cks1b, CKS1_HUMAN, Ckshs1, Cyclin dependent kinases regulatory subunit 1, Cyclin-dependent kinases regulatory subunit 1, NB4 apoptosis/differentiation related protein, PNAS 143, PNAS 16, PNAS 18
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.comEnlarge Image
Figure 1: Western Blot - CKS1 Cell Based ELISA Kit (A103545)
Western blot analysis of extracts from Jurkat cells, treated with serum 20% 15’, using Anti-CKS1 Antibody.
Protocol Diagram - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.comEnlarge Image
Figure 2: CKS1 Cell Based ELISA Kit (A103545)
A diagram showing the protocol for CKS1 Cell Based ELISA Kit.
Protocol Summary - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.comEnlarge Image
Figure 3: CKS1 Cell Based ELISA Kit (A103545)
A summary of the protocol for CKS1 Cell Based ELISA Kit.
Western Blot - Control Antibody - CKS1 Cell Based ELISA Kit (CB5933) - Antibodies.comEnlarge Image
Figure 4: Western Blot - CKS1 Cell Based ELISA Kit (A103545)
The mouse monoclonal antibody to GAPDH is used as a positive control inCKS1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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