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Anti-CISH / CIS Antibody (A87630)

Western Blot - Anti-CISH / CIS Antibody (A87630) - Antibodies.com Immunofluorescence - Anti-CISH / CIS Antibody (A87630) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CISH / CIS for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-CISH / CIS Antibody
Description
Rabbit polyclonal antibody to CISH / CIS.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:2,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 17-130 of human CISH (NP_659508.1).
Sequence
TGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVR
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
37 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-CISH / CIS Antibody (A87630) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-CISH / CIS Antibody (A87630)
Western blot analysis of extracts of 293T cells, using Anti-CISH / CIS Antibody (A87630) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 15s.
Immunofluorescence - Anti-CISH / CIS Antibody (A87630) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-CISH / CIS Antibody (A87630)
Immunofluorescence analysis of C6 cells using Anti-CISH / CIS Antibody (A87630) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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