Synthetic peptide within amino acids 550-600 of human CIP2A (NP_065941. 1).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate
Unconjugated
Purification
Antigen affinity purification.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Tris-Citrate/Phosphate Buffer, pH 7-8, with 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
General Notes
This antibody was affinity purified using the immunising peptide immobilized on solid support. Immunoglobulin concentration was determined by extinction coefficient: absorbance at 280 nm of 1. 4 equals 1.0 mg of IgG.
Synonyms
Cancerous inhibitor of PP2A, KIAA1524, p90 autoantigen, Protein CIP2A
Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Anti-CIP2A Antibody (A296475) was used for IP at 3 µg/mg lysate. CIP2A was also immunoprecipitated using another rabbit anti-CIP2A antibody, which recognizes a downstream epitope. For blotting immunoprecipitated CIP2A, this other antibody was used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
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