Anti-Choline Acetyltransferase Antibody [ARC0491] (A307812)

Name: Anti-Choline Acetyltransferase Antibody [ARC0491]
Brand: Antibodies.com
SKU: A307812

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This product is discontinued

Anti-Choline Acetyltransferase Antibody [ARC0491] (A307812) has been discontinued and is no longer available.

View all Choline Acetyltransferase Antibodies.

Specifications

Name

Anti-Choline Acetyltransferase Antibody [ARC0491]

Description

Rabbit monoclonal [ARC0491] antibody to Choline Acetyltransferase.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:2,000, ICC/IF: 1:50-1:200

Reactivity

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 649-748 of human CHAT (P28329).

Sequence

MSNRFVLSTSQVPTTTEMFCCYGPVVPNGYGACYNPQPETILFCISSFHSCKETSSSKFAKAVEESLIDMRDLCSLLPPTESKPLATKEKATRPSQGHQP

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0491

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Acetyl CoA choline O acetyltransferase, Acetyl CoA:choline O acetyltransferase, ChAT, CHOACTase, Choline acetylase, Choline O acetyltransferase, Choline O-acetyltransferase, CLAT_HUMAN, CMS1A, CMS1A2, EC 2.3.1.6, OTTHUMP00000019583, OTTHUMP00000019584

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Choline Acetyltransferase Antibody [ARC0491] (A307812)

Western blot analysis of extracts of SH-SY5Y cells, using Anti-Choline Acetyltransferase Antibody [ARC0491] (A307812) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunofluorescence - Anti-Choline Acetyltransferase Antibody [ARC0491] (A307812)

Immunofluorescence analysis of SH-SY5Y cells using Anti-Choline Acetyltransferase Antibody [ARC0491] (A307812) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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