Principle of Assay
Chloramphenicol Strip Test Kit (A327191) uses a competitive colloidal gold based format. The sample is added to the test well to allow the Chloramphenicol binding protein on the gold particles to engage with any Chloramphenicol present in the sample. Any gold particles that are not complexed with Chloramphenicol present in the sample will bind to the Chloramphenicol imprinted at the Test line (T-line), forming a signal (red line) at that position. If the Chloramphenicol binding protein on the gold particle has engaged with Chloramphenicol present in the sample, the gold particle will flow past the T-line and reach the Control line (C-line).
