Chloramphenicol ELISA Kit suitable for Animal tissues, honey, and feed samples. Reliably quantify 0.1 ppb (µg/kg). 100% Guaranteed.
Assay Type
Indirect (qualitative)
Principle of Assay
This ELISA kit is an indirect competitive enzyme immunoassay. Its main principle is that the unlabeled antibody is incubated in the presence of its antigen (sample). These bound antibody/antigen complexes are then added to an antigen-coated well. The plate is washed, so unbound antibody is removed. (The more antigen in the sample, the more Ag-Ab complexes are formed and so there are less unbound antibodies available to bind to the antigen in the well, hence "competition".) The secondary antibody, specific to the primary antibody, is added. This secondary antibody is coupled to the enzyme. A substrate is added, and remaining enzymes elicit a chromogenic or fluorescent signal. The reaction is stopped to prevent eventual saturation of the signal.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Animal tissues, honey, and feed samples.
Sensitivity
0.1 ppb (µg/kg)
Product Range
0.1-8.1 ppb
Storage
Store at 2-8°C for 12 months.
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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