Anti-Chk2 Antibody [ARC57076] (A306128)

Name: Anti-Chk2 Antibody [ARC57076]
Brand: Antibodies.com
SKU: A306128
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC57076] antibody to Chk2 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Chk2 Antibody [ARC57076]

Description

Rabbit monoclonal [ARC57076] antibody to Chk2.

Applications

WB, IHC

Dilutions

WB: 1:2,000-1:8,000, IHC: 1:100-1:500

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-180 of human Chk2 (O96017).

Sequence

GSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKR

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC57076

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

65 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

CDS 1, Cds1, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHEK 2, Chek2, Chk 2, CHK2 checkpoint homolog, CHK2 checkpoint homolog (S. pombe), CHK2_HUMAN, hCds1, HuCds 1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2, Serine/threonine-protein kinase Chk2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-Chk2 Antibody [ARC57076] (A306128)

Western blot analysis of HeLa, using Anti-Chk2 Antibody [ARC57076] (A306128) at 1:7,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 2: Western Blot - Anti-Chk2 Antibody [ARC57076] (A306128)

Western blot analysis of C2C12, using Anti-Chk2 Antibody [ARC57076] (A306128) at 1:7,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.

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Figure 3: Immunohistochemistry - Anti-Chk2 Antibody [ARC57076] (A306128)

Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-Chk2 Antibody [ARC57076] (A306128) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-Chk2 Antibody [ARC57076] (A306128)

Immunohistochemistry analysis of paraffin-embedded human testis using Anti-Chk2 Antibody [ARC57076] (A306128) at a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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