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Anti-Chk2 Antibody (A13908)

Western Blot - Anti-Chk2 Antibody (A13908) - Antibodies.com Western Blot - Anti-Chk2 Antibody (A13908) - Antibodies.com Immunofluorescence - Anti-Chk2 Antibody (A13908) - Antibodies.com Immunofluorescence - Anti-Chk2 Antibody (A13908) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Chk2 for WB and ICC/IF.

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Freight Charges
$40 to United States
Lead Time
5-8 Business Days

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+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com

Name
Anti-Chk2 Antibody
Description
Rabbit polyclonal antibody to Chk2.
Applications
WB, ICC/IF
Dilutions
WB: 1:100-1:500, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human Chk2 (NP_009125.1).
Sequence
MSRESDVEAQQSHGSSACSQPHGSVTQSQGSSSQSQGISSSSTSTMPNSSQSSHSSSGTLSSLETVSTQELYSIPEDQEPEDQEPEEPTPAPWARLWALQDGFANLECVNDNYWFGRDKSCEYCFDEPLLKRTDKYRTYSKKHFRIFREVGPKNSYIAYIEDHSGNGTFVNTELVGKGKRRPLNNNSEIALSLSRNKVFVFFDLTVDDQSVYPKALRDEY
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
65 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CDS 1, Cds1, Cds1 homolog, Checkpoint kinase 2, Checkpoint like protein CHK2, CHEK 2, Chek2, Chk 2, CHK2 checkpoint homolog, CHK2 checkpoint homolog (S. pombe), CHK2_HUMAN, hCds1, HuCds 1, LFS 2, LFS2, PP1425, RAD 53, RAD53, Rad53 homolog, Serine/threonine protein kinase Chk2, Serine/threonine-protein kinase Chk2
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Chk2 Antibody (A13908) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Chk2 Antibody (A13908)
Western blot analysis of extracts of Jurkat cells, using Anti-Chk2 Antibody (A13908) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-Chk2 Antibody (A13908) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Chk2 Antibody (A13908)
Western blot analysis of extracts of various cell lines, using Anti-Chk2 Antibody (A13908) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 60s.
Immunofluorescence - Anti-Chk2 Antibody (A13908) - Antibodies.comEnlarge Image
Figure 3: Immunofluorescence - Anti-Chk2 Antibody (A13908)
Immunofluorescence analysis of MCF-7 cells using Anti-Chk2 Antibody (A13908). DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-Chk2 Antibody (A13908) - Antibodies.comEnlarge Image
Figure 4: Immunofluorescence - Anti-Chk2 Antibody (A13908)
Immunofluorescence analysis of A549 cells using Anti-Chk2 Antibody (A13908).
Publishing research using Anti-Chk2 Antibody (A13908)? Please let us know so that we can list the citation on this page.

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